Homologous up-regulation of the 1,25 (OH)2 vitamin D3 receptor in rats |
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| Authors: | E M Costa D Feldman |
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| Institution: | 1. Communication Sciences and Disorders, The University of Western Ontario, London, ON, N6G 1H1, Canada;2. Brain and Mind Institute, The University of Western Ontario, London, ON, N6A 5B7, Canada;3. National Centre for Audiology, The University of Western Ontario, London, ON, N6G 1H1, Canada;4. Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON, N6A 5C1, Canada;5. Department of Psychology, The University of Western Ontario, London, ON, N6A 5C2, Canada;1. CAMH Research Imaging Centre and Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON, Canada;2. Institute of Medical Science, University of Toronto, Toronto, ON, Canada;3. Department of Psychiatry, University of Toronto, Toronto, ON, Canada;4. Department of Psychiatry and Behavioral Neurosciences, McMaster University, Hamilton, ON, Canada;5. TransBioTech, Lévis, QC, Canada;6. Toronto General Research Institute, University Health Network, Toronto, ON, Canada;1. Community Faculty, University of Minnesota Center for Spirituality and Healing, and Lipid Technologies LLC, P.O. Box 216, Austin, MN 55912, United States;2. Fellow, ASN, AAAS, SFRBM, 6100 Westchester Park Drive, College Park, MD 20740, United States;1. Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266000, China;2. Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao 266000, China;3. Department of Emergency, The Affiliated Hospital of Qingdao University, Qingdao 266000, China |
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| Abstract: | This study investigates the ability of vitamin D-metabolites to regulate 1,25(OH)2D3 receptors in vivo. Rats made vitamin D-deficient were treated with 1,25(OH)2D3 or vehicle for 1-5 days. In treated animals, receptors for 1,25(OH)2D3 in kidney increased dramatically compared with control levels. An increase in specific binding to 220% of control was seen after 2 doses of hormone, which reached to 336% after 5 days of treatment. Intestinal receptors increased to only 130% of control levels after 5 days of treatment. In vitamin D-replete animals, the difference between control and treated groups was slightly greater when endogenously occupied sites were measured by exchange (TPCK). However, significant changes were observed only after 4 days of hormone treatment. The data indicate that homologous up-regulation of the 1,25(OH)2D3 receptor occurs in vivo. The difference in response in kidney and in intestine suggests differential importance of up-regulation in various organs. |
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