|
|||||
|
|
Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues |
|
| Authors: | Pampusch M S Serie J R Osinski M A Seybold V S Murtaugh M P Brown D R |
| Institution: | a Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN USA b Macalester College, St. Paul, MN USA c Department of Neuroscience, University of Minnesota, Minneapolis, MN USA |
| Abstract: | This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific 3H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1–100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system. |
| Keywords: | Rat Pig Spleen Intestine mRNA-protein mismatch |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |