Dynamic properties of the active site of azurin studied by the temperature dependence of the optical spectrum |
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Authors: | Antonio Cupane Maurizio Leone Eugenio Vitrano Lorenzo Cordone |
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Institution: | (1) Istituto di Fisica dell'Università and GNSM-CISM, Via Archirafi 36, I-90123 Palermo, Italy |
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Abstract: | Summary We report the optical absorption spectra of azurin (Pseudomonas aeruginosa) in the temperature range 290-20 K. The samples used are protein aqueous solutions containing 65% (by Vol.) glycerol as cryoprotectant. The measured spectra are deconvoluted in gaussian components and the temperature dependence of the zeroth, first and second moment of the observed bands is analyzed using the harmonic Franck-Condon approximation for the coupling between electronic transitions and nuclear vibrations. The analysis provides information on the stereodynamic properties of the active site of this protein. The possible functional relevance of these results is also suggested. |
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Keywords: | Azurin Blue copper proteins Optical spectroscopy at cryogenic temperatures Protein dynamics Charge transfer transitions |
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