Callus production from leaf protoplasts of various cultivars of bean (Phaseolus vulgaris L.)

High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell colonies. When transferred to solid medium, the colonies gave rise to proliferating green calli. Deep green patches developed on these calli but failed to form shoots.Abbreviations NAA -NaphtaleneAcetic Acid - BA 6-Benzyl-Amino-purine - 2,4-D 2,4-Dichlorophenoxyacetic acid - GA₃ Gibberellic Acid - BCP BromoCresol Purple - MES 2-(N-Morpholino) EthaneSulphonic acid - MS Murashige and Skoog (1962)