Presynaptic dopaminergic properties of differentiated mouse embryonic stem cells |
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Authors: | Nakano Y Hirko A C Smith A D Oka M Dawson R Peris J Terada N Meyer E M |
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Institution: | Department of Pharmacology, College of Pharmacy, University of Florida, P.O. Box 100267, 1600 SW Archer, Gainesville, FL 32610, USA. |
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Abstract: | This study characterized the presynaptic dopaminergic properties of neuronally differentiated mouse embryonic stem (ES) cells. Approximately 30% of the ES cells expressed tyrosine hydroxylase (TH) immunoreactivity when co-cultured with PA6 cells. These cultures expressed high affinity, sodium-dependent dopamine uptake as well as depolarization-induced and calcium-dependent dopamine release of this transmitter. These and other important dopaminergic genes found expressed in these cultures by RT-PCR included Nurr1, vesicular monoamine transporter 2 (VMAT2), TH, dopamine transporter (DAT), and glial cell line-derived neurotrophic factor (GDNF) receptors c-Ret and GFRalpha1. These results demonstrate that differentiated ES cells have the presynaptic functions for maintaining dopaminergic homeostasis, which may be essential for their long-term use in restoring CNS levels of this transmitter. |
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