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Compact molten globule-like state of hUBF HMG Box1 at extremely low pH
Authors:Zhang Xuecheng  Zhang Jiahai  Li Xuan  Xu Junjie  Huang Hongda  Chen Quan  Wu Jihui  Shi Yunyu
Institution:School of Life Science, University of Science and Technology of China, People's Republic of China.
Abstract:Using far and near-UV CD, ANS fluorescence and 2D NMR spectroscopy, an acid-induced partly folded state (A state) at extremely low pH for hUBF HMG Box1 was identified and characterized. As compared to the native state (N), the A state has similar secondary structure, less compact pack with larger amounts of exposed hydrophobic surface, and narrower chemical shift dispersion in (1)H-(15)N HSQC spectrum, which implies that it is a molten globule (MG)-like species. On the other hand, substantial tertiary contacts and cooperative thermal denaturing transition indicate that the A state is closer-relative to the classic MG-to the native folded state. In addition, when the solution pH is adjusted to neutrality, the protein in the A state refolds to the native state easily. All these data suggest that the A state of hUBF HMG Box1 could represent a potential folding intermediate on protein folding pathway.
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