Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli |
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| Authors: | Liu Yan-Feng Ma Run-Lin Wang Si-Ling Duan Zi-Yuan Zhang Jin-Hai Wu Li-Jun Wu Chun-Fu |
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| Affiliation: | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China. |
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| Abstract: | The gene encoding a putative mature antitumor-analgesic peptide (AGAP) from the venom of the Chinese scorpion Buthus martensii Karsch was obtained by polymerase chain reaction (PCR) according to its cDNA sequence and expressed in Escherichia coli. While most of the recombinant AGAP was expressed in the form of insoluble inclusion body. The recombinant AGAP was purified to homogeneity by metal chelating affinity chromatography. Pharmaceutical tests showed that the recombinant AGAP has both analgesic and antitumor activities on mice. |
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