Rapid purification of two lipase isoenzymes from Candida rugosa |
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| Authors: | M. Luisa Rúa Antonio Ballesteros |
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| Affiliation: | (1) Unidad de Biocatálisis, Instituto de Catálisis, CSIC, 28049 Madrid, Spain;(2) Present address: Departamento de Biología Fundamental. Facultad de Ciencias, Universidad de Vigo, As Lagoas S/N, 32004 Ourense, Spain |
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| Abstract: | Summary We describe a two-step method for the purification of two lipases (lipases A and B) from C. rugosa. The purification procedure includes Phenyl-Sepharose and Sephacryl HR 100 chromatographies. The enzymes obtained were pure according to criteria of specific activity and neutral sugar content. |
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