Tissue-specific and developmental regulation of a gene encoding a low molecular weight sulfur-rich protein in soybean seeds |
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Authors: | Yeonhee Choi Ji Hoon Ahn Yang Do Choi and Jong Seob Lee |
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Institution: | (1) Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University, 151-742 Seoul, Korea;(2) Department of Agricultural Chemistry, Seoul National University, 441-744 Suwon, Korea |
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Abstract: | A gene corresponding to a cDNA clone, SE60, encoding a low molecular weight sulfur-rich protein in soybean seeds was isolated from a soybean genomic library and characterized at the nucleotide level. The SE60 gene is interrupted by an intervening sequence of 694 by in size. The 5 flanking region of the gene contained various regulatory sequences such as the RY repeat and CACA elements found in other seed protein genes of legumes. The SE60 gene encoded a pre-protein of 75 amino acids, having a signal sequence of 28 amino acids at the N-terminus. The mature protein of 47 amino acids was basic and cysteine-rich. Northern blot analysis suggested that the SE60 gene is expressed in a tissue-specific and developmentally regulated manner during soybean seed development. The SE60 genes form a small multigene family composed of about four members in the soybean genome. |
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Keywords: | Developmental regulation Soybean (Glycine max L ) Low molecular weight protein Multigene family |
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