Preservation of mouse embryos by two-step freezing |
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| Authors: | Maureen J. Wood John Farrant |
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| Affiliation: | 1. Medical Research Council Laboratory Animals Centre, Woodmansterne Road, Carshalton, Surrey SM5 4EF United Kingdom;2. Medical Research Council, Clinical Research Centre, Watford Road, Harrow, Middlesex HA1 3UJ United Kingdom |
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| Abstract: | Eight-cell mouse embryos in 1.5 M DMSO were preserved in LN2 by a two-step procedure. Fifteen minutes exposure at ?20 °C protected the embryos against damage during rapid cooling to -196 °C and during rapid warming and rapid dilution. Since survival was poor on slow warming it is suggested that the method permits the formation of some intracellular ice. |
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