| 外界高渗环境对枯草芽孢杆菌胞内自由脯氨酸含量的影响 |
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| 引用本文： | 杨清香,李用芳.外界高渗环境对枯草芽孢杆菌胞内自由脯氨酸含量的影响[J].氨基酸和生物资源,1999,21(3):9-11,29. |
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| 作者姓名： | 杨清香 李用芳 |
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| 作者单位： | 河南师范大学生物系!新乡453002 |
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| 摘 要： | 枯草芽孢杆菌(93151)在基本培养基中培养,可耐受高达8%NaCl浓度,而在10%NaCl条件下96h内未见生长。随胞外NaCl浓度的增加,其胞内自由脯氨酸的含量相应明显增加,在8%NaCl浓度下胞内脯氨酸含量可达2.944mg·g-1干细胞重,与无NaCl条件下相比,增加了1572%,而对照菌大肠杆菌仅可耐受4%NaCl浓度,并且在不同NaCl浓度下,胞内脯氨酸含量变化不明显。
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| 关 键 词： | 脯氨酸 高渗环境 枯草芽孢杆菌 相溶溶质 |
Influence of High Osmotic Environment on the Content of Free Proline in the Cells of Bacillus Subtilus |
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| Yang Qing xiang,Li Yong Fang,Li Xue mei,Liu Guosheng,Qin Yan.Influence of High Osmotic Environment on the Content of Free Proline in the Cells of Bacillus Subtilus[J].Amino Acids & Biotic Resources,1999,21(3):9-11,29. |
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| Authors: | Yang Qing xiang Li Yong Fang Li Xue mei Liu Guosheng Qin Yan |
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| Abstract: | Bacillus subtilus 93151 could grow in the minimal medium Containing 8% NaCl, but was completely inhibited by 10% NaCl. With the increasing of NaCl concentration, the content of free proline in the cells was increased. Under 8% NaCl, the content of proline increased to 2.944 mg·g -1 dry cell and increased by 1572%, compared to the cells grown in the medium without NaCl. The proline content in the cells of Escherichia coli grown in the medium containing 4% NaCl was not increased. |
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| Keywords: | proline high osmotic environment compatible solute Bacillus subtilus |
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