Specific binding of bovine immunoglobulins to Sepharose and Sephadex |
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| Authors: | Hiroshi Shimada Yuji Saito Teruaki Imada Yuji Inada |
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| Affiliation: | Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, Meguroku, Tokyo 152, Japan |
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| Abstract: | Bacteriophage T5 BglII/HindIII DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNASer1 and tRNASer2) with anticodons UGA and GGA, respectively. The main unusual structural feature of these tRNAs is the presence of extra non-basepaired nucleotides in the joinings of stem ‘b’ with stems ‘a’ and ‘c’. |
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| Keywords: | Bacteriophage T5 tRNA gene RNA/DNA hydridization DNA sequencing Isoacceptor tRNA |
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