Aminoacyl-tRNA synthetases: inter-site interaction as a possible proofreading mechanism |
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Authors: | Sergey Kh Degtyarev |
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Institution: | All-Union Research Institute of Molecular Biology, Koltsovo, Novosibirsk Region, 633159, USSR |
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Abstract: | Smooth muscle cell energetics of taenia caeci during relaxation, activity and maximal contraction were investigated using 31P-NMR. In relaxed muscle obtained in calcium-free medium, ATP], phosphocreatine] and sugar phosphate] were 4.4 mM, 7.7 mM and 2.8 mM, respectively. There was only a small difference in the energetics of spontaneously active and maximally contracted muscles, but under both conditions substantial changes occurred as compared with relaxed muscles. The internal pH in relaxed muscle was found to be 7.05, which acidified to 6.5 during contraction. The level of sugar phosphates was found to be not a limiting factor in energetics. |
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Keywords: | PCr phosphocreatine SP sugar phosphate NMR nuclear magnetic resonance |
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