Metabolic Basis of Detoxication: Metabolism of Functional Groups : Edited by W.B. Jakoby,J.R. Bend and J. Caldwell Academic Press: New York, 1982 357 + 18 pages. $42.00 |
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| Authors: | DV Parke |
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| Institution: | 1. Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Via S. Epifanio 14, Pavia, Italy;2. Istituto di Scienze Botaniche, Centro di Studio del CNR per la Biologia Cellulare e Molecolare delle Piante, Università di Milano, Italy;3. Biology Group DG XII Joint Research Centre, Ispra, Italy |
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| Abstract: | By inhibiting the α-like DNA polymerase, and therefore nuclear DNA synthesis, aphidicolin induces accumulation of suspension cultured carrot cells at the G1/S boundary of the cell cycle. After a 24-h treatment with the drug the accumulation is complete, affecting all the cycling cells (95% of the population). Upon removal of the inhibitor, all cycling cells immediately resume nuclear DNA synthesis and move synchronously throughout the S-phase. |
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| Keywords: | Synchronization Cultured plant cell Daucus carota DMSO dimethyl-sulfoxide ml semidented cells/100 ml culture |
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