Oesophagostomum radiatum: Glucose metabolism of larvae grown in vitro and adults grown in vivo |
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Authors: | RS Rew FW Douvres HJ Saz |
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Institution: | Ruminant Parasitic Disease Laboratory, Animal Parasitology Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, U.S.A.;Department of Biology, University of Notre Dame, Notre Dame, Indiana 46556, U.S.A. |
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Abstract: | Larval stages of Oesophagostomum radiatum grown in vitro and adults grown in vivo were incubated in complex media or in a simple salt solution containing radioactive glucose. Glucose disappearance and end product accumulation of third-stage larvae in a simple salt solution indicated that they excreted CO2 and acetic, propionic, and lactic acids. Larvae in third molt, fourth stage, and adults all excreted CO2, acetic, propionic, and lactic acids at twice the rate of third-stage larvae plus an additional product, methylbutyric acid. Carbon dioxide arose primarily from the 3 or 4 carbons of glucose. An anaerobic atmosphere (95% N2:5% CO2) had no apparent effect on metabolism. When incubation was done in complex media, isobutyric and 3-methylbutyric acids were seen as major excretion products (10 and 24%, respectively). However, these acids were quantitatively minor when incubations took place in simple salts-glucose medium (1 and 0–3%, respectively). |
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Keywords: | Nematode parasitic Glucose metabolism Cultivation Fatty acids branched-chain volatile |
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