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Partial reconstitution of the photosynthetic oxygen evolution system by rebinding of the 33-kDa polypeptide |
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| Authors: | Mitsue Miyao Norio Murata |
| Institution: | Department of Biology, University of Tokyo, Komaba, Meguro-ku, Tokyo 153, Japan |
| Abstract: | Treatment with 2.6 M urea of the Photosystem II particles depleted of two polypeptides of 24 kDa and 18 kDa completely released a polypeptide of 33 kDa and eliminated the oxygen-evolution activity. The 33-kDa polypeptide rebound to the urea-treated particles and partially reactivated the oxygen evolution. A quantitative analysis of the rebinding suggests tha there is a specific binding site for the 33-kDa polypeptide on the membrane surface. |
| Keywords: | 33-kDa polypeptide Oxygen evolution Photosystem II Photosynthesis (Spinach chloroplast) Chl chlorophyll DCIP 2 6-dichlorophenolindophenol Mes PS II photosystem II PAGE polyacrylamide gel electrophoresis |
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