Inhibitory effects of tannic acid on fatty acid synthase and 3T3-L1 preadipocyte |
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Authors: | Huijin Fan Dan Wu Weixi Tian Xiaofeng Ma |
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Affiliation: | College of Life Sciences, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China |
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Abstract: | Tannic acid is a hydrolyzable tannin that exists in many widespread edible plants with a variety of biological activities. In this study, we found that tannic acid potently inhibited the activity of fatty acid synthase (FAS) in a concentration-dependent manner with a half-inhibitory concentration value (IC50) of 0.14 μM. The inhibition kinetic results showed that the inhibition of FAS by tannic acid was mixed competitive and noncompetitive manner with respect to acetyl-CoA and malonyl-CoA, but uncompetitive to NADPH. Tannic acid prevented the differentiation of 3T3-L1 pre-adipocytes, and thus repressed intracellular lipid accumulation. In the meantime, tannic acid decreased the expression of FAS and down-regulated the mRNA level of FAS and PPARγ during adipocyte differentiation. Further studies showed that the inhibitory effect of tannic acid did not relate to FAS non-specific sedimentation. Since FAS was believed to be a therapeutic target of obesity, these findings suggested that tannic acid was considered having potential in the prevention of obesity. |
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Keywords: | Ac-CoA, acetyl CoA ACP, acyl carrier protein DH, dehydrase DMSO, dimethyl sulfoxide ER, enoyl reductase FAS, fatty acid synthase FBS, fetal bovine serum IC50, half-inhibitory concentration KR, ketoacyl reductase KS, ketoacyl synthase Mal-CoA, malonyl-CoA MAT, malonyl/acetyltransferase MTT, 3-4, 5-dimethylthiazol-2-yl-2, 3-diphenyl tetrazolium bromide PBS, phosphate-buffered saline PPAR, peroxisome proliferator-activated receptors TE, thioesterase |
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