| 用分子生物学技术对草菇进行菌株鉴别 |
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| 引用本文： | 陈明杰 赵绍惠 张树庭. 用分子生物学技术对草菇进行菌株鉴别[J]. 菌物学报, 1996, 15(2) |
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| 作者姓名： | 陈明杰 赵绍惠 张树庭 |
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| 作者单位： | 食用菌遗传育种农业部部级重点实验室，上海农业科学院食用菌研究所 上海 201106；香港中文大学生物系 沙田 香港 |
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| 基金项目： | UPGC Earmarked Great(CUHK 21/91)基金 |
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| 摘 要： | 利用AP-PCR和RAPD技术对三个草菇菌株进行鉴别,其结果与用草菇菌株V34基因文库中的中等重复序列为探针进行限制性内切酶长度多态性分析(RFLP),及对编码核糖体5.8SrRNA的DNA(rDNA)进行PCR扩增后的产物进行限制性内切酶长度多态性分析(PCR-RFLP)的结果相一致。这一结果显示出用这四种方法对草菇菌株进行鉴别具有相似的效果。同时用这四种方法构建的分子生物学标记显示出这三个菌株中的两个菌株在遗传上有着较大程度的相似性。
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| 关 键 词： | 草菇 菌株鉴定 限制性内切酶长度多态性 随机引物多聚酶链式反应 随机扩增 DNA多态性 PCR扩增产物限制性内切酶长度多态性分析 |
CHARACTERIZED VOLVARIELLA VOLVACEA CULTIVATED STRAINS BY MOLECULAR TOOLS |
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| Chen Mingjie. CHARACTERIZED VOLVARIELLA VOLVACEA CULTIVATED STRAINS BY MOLECULAR TOOLS[J]. Mycosystema, 1996, 15(2) |
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| Authors: | Chen Mingjie |
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| Abstract: | Three Volvariella volvacea cultivated strains were characterized using AP-PCR and RAPD. The results were compared with RFLPs (restriction fragment length polymorphism) using the moderately repetitive probes obtained from constructed partial genomic library of V. volvacea strain V34, and another DNA profiles obtianed by PCR-RFLPs, the restriction fragment length polymorphism in the PCR amplified ribosomal RNA sequence. The results demonstrated that the moderately repetitive probes type strains as effectively as other DNA fingerprinting methods. Also, the four types of molecular markers consistently suggest that two of three V. volvacea strains are genetically related. |
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| Keywords: | Volvariella volvacea strain type RFLP AP-PCR RAPD PCR-RFLP. |
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