|
|||||
|
|
| 小菜蛾高效Bt菌株的分离、生化特性及基因型鉴定 | |
| 引用本文： | 朱勋,吴青君,张友军,刘春光,刘岩林,余亚军,景伟,薛原,杨峰山.小菜蛾高效Bt菌株的分离、生化特性及基因型鉴定[J].昆虫知识,2011,48(2):285-290. |
| 作者姓名： | 朱勋 吴青君 张友军 刘春光 刘岩林 余亚军 景伟 薛原 杨峰山 |
| 作者单位： | 1. 黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,哈尔滨,150080;中国农业科学院蔬菜花卉研究所,北京,100081 2. 中国农业科学院蔬菜花卉研究所,北京,100081 3. 黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,哈尔滨,150080 |
| 基金项目： | 公益性行业(农业)科研专项，国家自然科学基金，黑龙江省自然科学基金，哈尔滨市科技创新人才研究专项，黑龙江省教育厅面上项目 |
| 摘 要： | 从哈尔滨田间采集死亡小菜蛾Plutella xylostella（L.）幼虫,从中分离出10株苏云金芽孢杆菌Bacillus thuringiensis。对小菜蛾幼虫室内生物测定结果表明,各菌株对小菜蛾幼虫的死亡率均在85%以上,其中DBW902毒力最强,48h的LC50为13.99mg/L。菌株cry/cyt基因检测表明,所有菌株均含有cry1A或cry2A基因,这与其高毒力的特性基本吻合。生化检测与分析表明,菌株DBW904、DBW93、DBW962与已报道的苏云金芽孢杆菌山东亚种B.thuringiensis subsp.shandongiensis的生化特性一致,但是其它菌株的生理生化特性与已报道菌株有区别。 |
| 关 键 词： | 苏云金芽孢杆菌 鉴定 杀虫活性 cry/cyt基因 |
Isolation, biochemical and cry-type gene characterization of Bacillus thuringiensis strains with high toxic to Plutella xylostella |
|
| ZHU Xun,WU Qing-Jun,ZHANG You-Jun,LIU Chun-Guang,LIU Yan-Lin,YU Ya-Jun,JING Wei,XUE Yuan,YANG Feng-Shan.Isolation, biochemical and cry-type gene characterization of Bacillus thuringiensis strains with high toxic to Plutella xylostella[J].Entomological Knowledge,2011,48(2):285-290. | |
| Authors: | ZHU Xun WU Qing-Jun ZHANG You-Jun LIU Chun-Guang LIU Yan-Lin YU Ya-Jun JING Wei XUE Yuan YANG Feng-Shan |
| Institution: | ZHU Xun1,2 WU Qing-Jun2 ZHANG You-Jun2 LIU Chun-Guang1 LIU Yan-Lin1 YU Ya-Jun1 JING Wei1 XUE Yuan1 YANG Feng-Shan1(1.Key Laboratory of Microbiology of Heilongjiang Province,College of Life Science,Heilongjiang University,Harbin 150080,China,2.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
| Abstract: | The insecticidal activities of ten strains of Bacillus thuringiensis isolated from diseased Plutella xylostella(L.) larvae were characterized.All strains caused > 80% larval mortality and the strain DBW902 had the highest insecticidal activity with an LC50 of 13.99 mg/L at 48 h.The biochemical characteristics and cry/cyt genes of each strain were determined.High insecticidal activity coincided with the detection of at least one cry1A or cry2A family gene.The biochemical characteristics of the strains,DBW904... |
| Keywords: | Bacillus thurigiensis identification insecticidal activity cry/cyt genes |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |