Angiopoietin-related growth factor (AGF) supports adhesion, spreading, and migration of keratinocytes, fibroblasts, and endothelial cells through interaction with RGD-binding integrins |
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Authors: | Zhang Yueqing Hu Xiaobo Tian Ruiyang Wei Wangui Hu Wei Chen Xia Han Wei Chen Huayou Gong Yi |
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Institution: | Shanghai Institute for Biological Sciences, Graduate school of Chinese Academy of Sciences, Shanghai, PR China. |
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Abstract: | Angiopoietin-related growth factor (AGF) is a newly identified member of angiopoietin-related proteins (ARPs)/angiopoietin-like proteins (Angptls). AGF has been considered as a novel growth factor in accelerating cutaneous wound healing, as it is capable of stimulating keratinocytes proliferation as well as angiogenesis. But in our paper, we demonstrate that AGF stimulates keratinocytes proliferation only at high protein concentration, however, it can potently promote adhesion, spreading, and migration of keratinocytes, fibroblasts, and endothelial cells. Furthermore, we confirm that the adhesion and migration cellular events are mediated by RGD-binding integrins, most possibly the alpha(v)-containing integrins, by in vitro inhibition assays using synthetic competitive peptides. Our results strongly suggest that AGF is an integrin ligand as well as a mitogenic growth factor and theoretically participates in cutaneous wound healing in a more complex mechanism. |
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Keywords: | Angiopoietin-related growth factor RGD-binding integrins Keratinocyte Fibroblast Endothelial cell Cell proliferation Cell adhesion Cell spreading Cell migration |
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