首页 | 本学科首页   官方微博 | 高级检索  
     


Activity of Plasmid Replicons in CAULOBACTER CRESCENTUS : Rp4 and Cole1
Authors:O'neill E A  Berlinberg C  Bender R A
Affiliation:Department of Cellular and Molecular Biology, Division of Biological Sciences, University of Michigan, Ann Arbor, Michigan 48109.
Abstract:The RP4 replicon was detected as covalently-closed circular DNA in Caulobacter crescentus strains into which it had been transferred from Escherichia coli. RP4-mediated transfer of ColE1-associated markers into C. crescentus occurred, but only as the result of transposon-mediated events. Both transposition of a ColE1-associated marker onto RP4 and cointegration of ColE1 with RP4 were observed. Chimeric plasmids containing both a ColE1 and an RP4 origin of replication were stably maintained in C. crescentus , but similar plasmids lacking the RP4 origin of replication were not stably maintained in C. crescentus. Thus we show that the ColE1 replicon cannot be maintained in C. crescentus unless it is covalently linked to another replicon, such as RK2, that can be maintained.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号