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Isolation of a sequence common to A- and B-chromosomes of rye (Secale cereale) by microcloning |
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| Authors: | Michael J Sandery John W Forster Simon R Macadam Richard Blunden R Neil Jones Stephen D M Brown |
| Institution: | (1) Centre for Plant Biotechnology, Bond University, 4229 Gold Coast, Queensland, Australia;(2) Genetics and Crop Biotechnology Group, Department of Agricultural Sciences, University College of Wales, Aberystwyth, SY23 3DD Dyfed, Wales, UK;(3) Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Paddington, W21PG London, UK |
| Abstract: | The techniques of microdissection and microcloning have been applied to the isolation of B-chromosome DNA from rye. We have identified a DNA sequence on the rye B-chromosome which is homologous to an A-chromosome sequence, and which is dispersed and moderately repeated on the A- and B-chromosomes. This demonstrates that the rye B-chromosome is heterogeneous in the nature of its DNA sequence composition, containing sequences which are present on the A-chromosomes in addition to those not present on the A-chromosomes. |
| Keywords: | B-chromosome microcloning rye evolution |
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