| 丹参胁迫诱导基因SmSIP1的表达特征分析 |
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| 引用本文： | 赵燕燕,王喆之. 丹参胁迫诱导基因SmSIP1的表达特征分析[J]. 植物生理学通讯, 2008, 44(6) |
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| 作者姓名： | 赵燕燕 王喆之 |
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| 作者单位： | 陕西师范大学生命科学学院,教育部药用植物资源与天然药物化学重点实验室,西安,710062 |
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| 基金项目： | "十一五"科技史撑计划 |
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| 摘 要： | 从丹参EST库中筛选到一个胁迫诱导蛋白基因，命名为SmS1P1，其序列全长296bp，编码80个氨基酸。生物信息学预测表明SmS1P1是一个亲水的，不具有跨膜结构域，包含一个N-端信号肽和多个可能的磷酸化位点的蛋白。实时荧光定量PCR分析显示，SmS1P1在根中的表达量高于茎和叶，并且受ABA和干旱的诱导，推测其可能参与根部的胁迫应答反应。
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| 关 键 词： | 胁迫诱导蛋白 丹参 生物信息学 基因表达 |
Expression Characteristics of SmSIP1 in Salvia miltiorrhiza Bunge |
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| ZHAO Yan-Yan,WANG Zhe-Zhi. Expression Characteristics of SmSIP1 in Salvia miltiorrhiza Bunge[J]. Plant Physiology Communications, 2008, 44(6) |
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| Authors: | ZHAO Yan-Yan WANG Zhe-Zhi |
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| Affiliation: | ZHAO Yah-Yan,WANG Zhe-Zhi~* Key Laboratory of Ministry of Education for Medicinal Resource , Natural Pharmaceutical Chemistry,College of Life Sciences,Shaanxi Normal University,Xi'an 710062,China |
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| Abstract: | A stress induced gene was selected from EST library of Salvia miltiorrhiza Bunge.The cDNA sequence named SmSIP1 is 296 bp long,encoding a protein of 80 amino acid residues.Bioinformatics analysis showed that the deduced SmSIP1 was a hydrophilic,non-transmembrane protein with a N-terminal signal peptides and several probable phosphorylation sites.Real-time fluorescent quantitative PCR was used to detect the expression pattern of SmSIP1 in different organs of S.miltiorrhiza.The results showed that the express... |
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| Keywords: | stress-induced protein Salvia miltiorrhiza Bunge bioinformatics gene expression |
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