Biomass Measurement of Methanogens in the Sediments of Tokyo Bay Using Archaeol Lipids |
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Authors: | Michinari Sunamura Yosuke Koga Kouichi Ohwada |
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Institution: | (1) Marine Microbiology Division, Ocean Research Institute, University of Tokyo, Nakano-ku, Tokyo 164-8639, Japan, JP;(2) Department of Chemistry, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu 807-8555, Japan, JP |
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Abstract: | An archaeal ether-linked lipid, archaeol, was determined to be a biomass indicator for methanogens both in the laboratory
enriched culture and in marine sediments. The archaeol measurement method described by Ohtsubo et al. in 1993 was modified
and applied to marine sediments. We compared the amount of archaeol with the cell number of methanogens or methane concentration
in laboratory enriched culture of methanogens from marine sediment. Good correlations were obtained as follows: (Methane,
mmol) = 11.2 × (Archaeol, mg): r= .996 or (Cell number) = 1.13 × 1011× (Archaeol, mg): r= .995, respectively. In the sediments of Tokyo Bay, archaeol was measured from approximately 46 to 561 ng/dry g sediment
at the entrance to 267 to 4160 ng/dry g sediment at the innermost area. Using the coefficient from the laboratory experiment,
these data corresponded to cell numbers of 5.2 × 106 to 4.7 × 108/dry g sediment. These values were 1 or 2 orders of magnitude higher than those obtained by culture methods in previous studies.
Although dead or decomposed cells might be detected, archaeol measurement is useful for estimating the biomass of methanogens
because of the good correlation between methane concentration and archaeol content in marine environments. In this study,
we found a correlation of (Methane, mmol) = 0.012 × (Archaeol, mg): r= .932, n= 17 in marine sediments.
Received December 21, 1998; accepted June 16, 1999 |
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Keywords: | : Archaeol biomass measurement methanogen sediment Tokyo Bay |
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