EDC4 interacts with and regulates the dephospho-CoA kinase activity of CoA synthase |
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| Authors: | Daria Gudkova Ganna Panasyuk Ivan Nemazanyy Alexander ZhyvoloupPascale Monteil Valeriy FilonenkoIvan Gout |
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| Affiliation: | a State Key Laboratory of Molecular and Cellular Biology, Institute of Molecular Biology and Genetics, Kyiv 03680, Ukraine
b Department of Structural and Molecular Biology, University College London, London WC1E 6BT, UK |
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| Abstract: | Coenzyme A synthase (CoAsy) is a bifunctional enzyme which facilitates the last two steps of Coenzyme A biogenesis in higher eukaryotes. Here we describe that CoAsy forms a complex with enhancer of mRNA-decapping protein 4 (EDC4), a central scaffold component of processing bodies. CoAsy/EDC4 complex formation is regulated by growth factors and is affected by cellular stresses. EDC4 strongly inhibits the dephospho-CoA kinase activity of CoAsy in vitro. Transient overexpression of EDC4 decreases cell proliferation, and further co-expression of CoAsy diminishes this effect. Here we report that EDC4 might contribute to regulation of CoA biosynthesis in addition to its scaffold function in processing bodies.Structured summary of protein interactionsCoAsyphysically interacts with EDC4 by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3) |
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| Keywords: | CoA synthase CoA Processing bodies EDC4 Decapping Mitochondria |
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