Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG |
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| Authors: | Shuntaro TsukamotoShuya Yamashita Yoon Hee KimMotofumi Kumazoe Yuhui HuangKoji Yamada Hirofumi Tachibana |
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| Affiliation: | a Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
b Laboratory of Functional Food Design, Department of Functional Metabolic Design, Bio-Architecture Center, Kyushu University, Fukuoka 812-8581, Japan |
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| Abstract: | (−)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O2 levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O2. In summary, 67LR expression is highly sensitive to O2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O2 partial pressure. |
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| Keywords: | EGCG, (&minus )-epigallocatechin-3-O-gallate 67LR, 67-kDa laminin receptor HIF-1a, hypoxia-inducible factor 1 alpha MRLC, myosin II regulatory light chain ROS, reactive oxygen species |
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