Isolation and biochemical characterization of a new NADH oxidase from Lactobacillus brevis |
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Authors: | Hummel Werner Riebel Bettina |
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Affiliation: | (1) Institut für Enzymtechnologie der Heinrich-Heine-Universität, Forschungszentrum Jülich, Wilhelm-Johnen-Str., D-52426 Jülich, Germany |
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Abstract: | A new NADH oxidase, useful for the regeneration of NAD+, was isolated and characterized from Lactobacillus brevis. In crude extracts the activity was from 10–15 U mg–1. After purification by four chromatographic steps, an activity of 116 U mg–1 was obtained with 14% yield. Highest activity was from pH 5.5–7 and at 40°C. The enzyme requires dithiothreitol to prevent oxidative deactivation. The Km value for NADH was 24 M. |
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Keywords: | chiral alcohols Lactobacillus brevis NADH oxidase oxidation |
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