H2O2对红细胞抗原性与LPO影响的FCM研究

用流式细胞计（flow cytometry,FCM）测定H₂O₂损伤后的红细胞（RBC）与IgG的结合能力，并直接检测细胞自发荧光的变化，以研究H₂O₂对RBC抗原性和脂质过氧化（LPO）荧光产物生成的影响.结果表明RBC抗原性和自发荧光变化与H₂O₂浓度和作用时间有关，抗原性变化对H₂O₂更敏感，比引起自发荧光明显增强所需H₂O₂浓度低两个数量级；还发现了RBC抗原性和自发荧光的变化与细胞的散射光有相关性.

Studies on the Effect of H2O2 on the Antigenicity and LPO of RBC by FCM

Using flow cytometry(FCM) to measure IgG binding to red blood cells(RBC) exposed to H₂O₂ and their autofluorescence directly, in order to study the effect of H₂O₂ on the antigenicity and the production of fluorescent product of LPO of RBC. The results showed that the changes of both antigenicity and autofluorescence were dependent on the H₂O₂ concentration and the time of RBCs exposed to H₂O₂, and the change of antiginicity was more susceptible to H₂O₂ than that of autofluorescence. At 10² fold lower H₂O₂ concentration than that made autofluorescence significantly increase, IgG bond to RBC. Furthermore, it was found that the changes of antigenicity and autofluorescence were related to forward scatter (FSC).