Serological detection of ryegrass mosaic virus and ryegrass seed-borne virus |
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| Authors: | IRENE B CHESTER S A HILL DAPHNE M WRIGHT |
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| Institution: | Agricultural Development and Advisory Service, Plant Virus Unit, Brooklands Avenue, Cambridge, CB2 2DR |
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| Abstract: | Ryegrass mosaic virus (RMV) was reliably detected in both perennial (S24) and Italian (S22) ryegrass, by enzyme-linked immunosorbent assay (ELISA) when plants had been infected for 8 wk. ELISA detected more infections in field-grown perennial ryegrass cv. Premo than either visual assessment or electron microscopy. However, with plants of Italian ryegrass cultivars only recently infected with RMV, positive reactions were more difficult to separate from the reactions of RMV-free plants, which varied considerably with cultivar, some giving high absorbance values. Immunosorbent electron microscopy showed that the RMV antiserum also contained antibodies to ryegrass seed-borne virus (RGSV), suggesting that these high values were caused by RGSV infection in the material tested. |
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