Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro |
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Authors: | M Ziv H Lilien-Kipnis |
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Institution: | (1) Agricultural Botany and the Warburg Center for Biotechnology in Agriculture The Hebrew University of Jerusalem, Rehovot 76100, Israel Fax: +972–8-9467763 e-mail: meira@agri.huji.ac.il, IL;(2) Ornamental Horticulture Agricultural Research Organization, Bet-Dagan 50250, Israel, IL |
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Abstract: | Bulbs, corms and other subterranean storage organs are commonly used as explant source material for the establishment of
geophytes in vitro. The inflorescence stalk was found to be a good alternative source of explants to overcome explant contamination
originating from underground storage organs. Inflorescence explants of Allium, Dichelostemma, Eucrosia, Gladiolus, Haemanthus, Hyacinthus, Narcissus, Nerine and Ornithogalum were used to establish cultures in vitro. The regeneration potential of the inflorescence was compared with regeneration
from bulb twin scales or from apical buds isolated from corms. Gladiolus (Iridaceae) explants isolated from the floral stem just below the expanding florets, still enclosed in the bracts, were highly
regenerative in the presence of naphthalene acetic acid (NAA) and kinetin. In the presence of 2,4-dichlorophenoxyacetic acid
and benzyl aminopurine (BA) in the medium, explants isolated from the tissue at the junction between the peduncle and the
pedicels of a young Nerine (Amaryllidaceae) inflorescence regenerated several buds. The scapes of young unemerged inflorescences taken from sprouting
bulbs of Narcissus (Amaryllidaceae), following a 15 °C storage treatment, regenerated buds in the presence of NAA, BA, elevated phosphate and
adenine sulfate in the medium. The number of buds regenerated depended on the location on the scape from which the explant
was isolated, and on the duration of the 15°C treatment. In Allium (Alliaceae), capitulum tissue between the flower pedicels regenerated buds. Explants excised from the peduncle, as well as
the pedicel-peduncle junction of Dichelostemma (Alliaceae), Ornithogalum, Hyacinthus (Hyacinthaceae) and Eucrosia (Amaryllidaceae) regenerated several buds in each type of explant. In the case of Haemanthus (Amaryllidaceae), pedicel-peduncle junction explants regenerated buds only when excised from inner whorl florets. Propagation
protocols and the potential use of expediently isolated inflorescence explants for efficient micropropagation of geophytes
are discussed.
Received: 1 September 1999 / Revision received: 13 December 1999 / Accepted: 13 December 1999 |
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Keywords: | Bud regeneration Bulbs Corms Pedicel Peduncle |
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