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Structural studies of the extracellular polysaccharides of Rhizobium japonicum strains 71A,CC708, and CB1795
Authors:William F Dudman
Institution:Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, P.O. Box 1600, Canberra City, A.C.T. 2601 Australia
Abstract:Each of the serologically related, title strains produced extracellular polysaccharides that were mixtures of an acidic polysaccharide and smaller proportions of a glucan. These were separated by column chromatography and the structures of the acidic polysaccharides investigated by alkylation, specific sequential degradation, and periodate oxidation in conjunction with 1H-n.m.r. spectroscopy. The polysaccharides from the three strains appear to be identical rhamno-4-O-methyl-glucuronans, having the following tetrasaccharide repeating-unit.
/></figure> The molecular weight of the polysaccharides of strains CC708 and CB1795 was of the order of 65,000–70,000.</td>
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