Infrared absorption and raman scattering of sulfate groups of heparin and related glycosaminoglycans in aqueous solution |
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Authors: | Franco Cabassi Benito Casu Arthur S Perlin |
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Institution: | Italian Research Council, Istituto Chimica Macromolecole, Milan Italy;Istituto di Chimica e Biochimica G. Ronzoni, Milan Italy;Department of Chemistry, McGill University, Montreal Canada |
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Abstract: | The i.r. spectra for aqueous solutions of sulfated glycosaminoglycans and model compounds in the transmittance “window” region of the solvent (1400-950 cm?1) are dominated by the strong and complex absorption centered at ~1230 cm?1 and associated with the antisymmetric stretching vibrations of the SO groups. Primary and secondary O-sulfate groups absorb at somewhat higher frequencies (1260-1200 cm?1) than N-sulfates (~1185 cm?1). Each sulfate band lends itself to quantitative applications, especially within a given class of sulfated polysaccharide. Laser-Raman spectra of heparin and model compounds have been obtained in aqueous solution and in the solid state. The most-prominent Raman peak (at ~1060 cm?1) is attributable to the symmetrical vibration of the SO groups, with N-sulfates emitting at somewhat lower frequencies (~1040 cm?1) than O-sulfates. The Raman pattern in the 950-800 cm?1 region (currently used in the i.r. for distinguishing between types of sulfate groups) also involves vibrations that are not localized only in the COS bonds. |
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