An improved synthesis of 6-azathymidine |
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Authors: | George T Shiau William H Prusoff |
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Institution: | Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06510 U.S.A. |
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Abstract: | The trisaccharides panose and isopanose were prepared in good yield from enzymic hydrolyzates of pullulan. Pullulan was hydrolyzed by the purified alpha amylase preparation of Thermoactinomyces vulgaris R-47. The digest was applied to a carbon-Celite column and eluted with a linear gradient of 1-propanol from 0 to 5%. From the trisaccharide fractions eluted, panose was prepared in about 70% yield. Pullulan was also hydrolysed by purified isopullulanase (EC 3.2.1.57 pullulan 4-glucanohydrolase) of Aspergillus niger ATCC-9642, and isopanose was prepared in about 90% yield by using the same technique as that for the preparation of panose. |
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Keywords: | To whom communication should be addressed |
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