Structural analysis and ensymic solubilization of barley endosperm cell-walls |
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Authors: | G.Murray Ballance David J. Manners |
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Affiliation: | Department of Brewing and Biological Sciences, Heriot-Watt University, Edinburgh EHI 1HX Great Britain |
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Abstract: | Barley endosperm cell-walls were prepared and analysed. The-carbohydrate portion, which constituted most of the wall material, consisted of 10 % of l-arabinose, 13% Of d-xylose, 74% of d-glucose and 2.5% of d-mannose. Mixed-linkage β-d-glucan represented 70-72% of this material; the remaining 2-4% Of d-glucose maybe present as cellulose and glucomannan. Water and alkali-extracted β-d-glucans contained similar ratios of (1 → 3)- to (1 → 4)-Linkages, namely 3 to 7. The walls, which had a protein content of approximately 5 %, contained unidentfied, alkali-labile linkages. An endo-(1 → 3)β-d-glucanase from malted barley, and a fungal endo-(1 → 4)-β-d-glucanase, caused extensive solubilization of the wall polysaccharides. |
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