Reduction of nonspecific binding proteins to self-assembled monolayer on gold surface |
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Authors: | Furuya Minoru Haramura Masayuki Tanaka Akito |
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Institution: | Chemistry Department, Reverse Proteomics Research Institute Co. Ltd, 2-6-7 Kazusa-Kamatari, Chiba 292-0818, Japan. |
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Abstract: | We developed a gold coated glass chip bearing a poly(ethyleneglycol) (PEG) type compound as hydrophilic spacer for surface plasmon resonance studies, which enabled adequate estimation of K(d) value between FK506 and FKBP12 not only using purified FKBP12 (K(d)=22 nM) but also using Escherichia coli lysate expressing FKBP12 (K(d)=15 nM). These results indicated effectiveness of the PEG spacer for reduction of nonspecific interactions. Chemical stability and simple surface-structure of the novel chip are also attractive. |
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