In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension. |
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Authors: | R E Wellinger and J M Sogo |
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Abstract: | By the use of psoralen crosslinking and primer extension, a method was developed which allows the analysis of chromatin structure in vivo. Using a yeast minichromosome, >9 nucleosomes were mapped with a resolution of at least +/-30 bp. |
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