Glycogen in Methanothrix |
| |
Authors: | P Pellerin B Gruson G Prensier G Albagnac P Debeire |
| |
Institution: | (1) Institut National de la Recherche Agronomique, Station de Technologie Alimentaire, B.P. 39, F-59651 Villeneuve d'Ascq Cedex, France;(2) Institut National de la Santé et de la Recherche Médicale U 42, B. P. 39, F-59651 Villeneuve d'Ascq Cedex, France |
| |
Abstract: | An intracellular glycogen was purified and characterized from the acetoclastic bacteria Methanothrix str. FE, its average chain length was about 13 glucose residues. Acetyl-CoA was shown to be synthesized by the action of acetate thiokinase; in addition pyruvate synthase, phosphoenolpyruvate synthetase and enzymes of gluconeogenesis were detected in cell extracts. For glycogen synthase activity, both adenosine diphosphate glucose and uridine diphosphate glucose were used as glycosyl donors, apparent K
m were, respectively, 8 M for ADPGlc and 625 M for UDPGLe, at the opposite the V
m were the same for both precursors. This was in accordance with competition experiments and strongly suggested that only one glucosyl transferase was involved and that ADPGlc was the physiological glycosyl donor in Methanothrix str. FE. In addition branching enzyme activity ( 1-4-glucan-6-glucosyl transferase) was detected in cell extracts.Abbreviations ADPGlc
adenosine diphosphate glucose
- UDPGlc
uridine diphosphate glucose |
| |
Keywords: | Methanothrix Archaebacteria Gluconeogenesis Glycogen Glycogen synthase |
本文献已被 SpringerLink 等数据库收录! |
|