Genetic control of variation in human gingival fibroblast proliferation rate |
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| Authors: | George H. Cockey Joann A. Boughman Emily L. Harris Thomas M. Hassell |
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| Affiliation: | (1) Department of Anatomy, Baltimore College of Dental Surgery, 21201 Baltimore, Maryland;(2) Division of Genetics, University of Maryland School of Medicine, 21201 Baltimore, Maryland;(3) Department of Epidemiology, The Johns Hopkins University School of Hygiene and Public Health, 21205 Baltimore, Maryland;(4) Department of Periodontology, J. Hillis Miller Health Center, The University of Florida College of Dentistry, Box J-434, 32610 Gainesville, Florida |
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| Abstract: | Summary Logarithmic proliferation rate (Days 1 to 6) of gingival fibroblasts derived from 15 pairs of monozygotic (MZ) and 9 pairs of dizygotic (DZ) human twins was compared under optimal and suboptimal growth conditions. Cell proliferation rates exhibited considerable variability among strains. For Caucasian donors (13 MZ, 6 DZ pairs) DZ twins demonstrated significantly greater (P<0.01) within-pair variance in cell proliferation rate compared to MZ twins when evaluated under optimal growth conditions. Heritability analysis indicated strong genetic control of proliferation rate of human gingival fibroblasts (HGF) under optimal growth conditions (1.0±0.67), whereas proliferation rate of HGF under suboptimal growth conditions revealed less genetic control (0.42±0.61). These findings emphasize the importance of carefully matching control and test HGF in assays dependent on cellular proliferation. This work was supported by grants DE-06671 and DE-07841 from the National Institutes of Health. |
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| Keywords: | fibroblasts proliferation genetics gingiva |
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