Differentiated structure and function of primary cultures of monkey oviductal epithelium |
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Authors: | M Rajagopal T L Tollner W E Finkbeiner G N Cherr J H Widdicombe |
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Institution: | (1) Department of Physiology & Membrane Biology, University of California-Davis, 95616 Davis, California;(2) Department of Reproductive Biology, University of California-Davis, 95616 Davis, California;(3) Department of Pathology, University of California-Davis, 95616 Davis, California |
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Abstract: | Summary We have established well-differentiated, polarized cultures of monkey oviductal epithelium. Oviductal epithelial cells were
isolated by protease digestion and plated on collagen-coated, porous cell culture inserts. About 5 d after plating, cells
developed detectable transepithelial electrical resistance of up to 2000 Ω.cm2 (an index of tight junction formation) and transepithelial voltages of up to 20 mV (an index of vectorial transepithelial
ion transport). Measurements of short-circuit current in Ussing chambers indicated that active secretion of Cl was the major
transepithelial active ion transport process, and that this was stimulated by elevation of either cAMP or Ca. Furthermore,
estimates of the volume of mucosal liquid were consistent with Cl secretion mediating fluid secretion. Various microscopical
methods showed that the cultures were densely ciliated and contained mature secretory cells. Transport across the oviductal
epithelium determines the composition of the oviductal fluid, and the study of the relevant transport processes will be greatly
enhanced by well-differentiated cultures of oviductal epithelium of the kind established here. |
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Keywords: | chloride secretion purinergic agonists short-circuit current transepithelial electrical resistance |
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