Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells |
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Authors: | Bankapur Aseefhali Krishnamurthy R Sagar Zachariah Elsa Santhosh Chidangil Chougule Basavaraj Praveen Bhavishna Valiathan Manna Mathur Deepak |
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Institution: | Centre for Atomic and Molecular Physics, Manipal University, Manipal, India. |
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Abstract: | We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 μg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 μg/ml. |
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