Effect of amino acid substitutions on the candidacidal activity of LFampin 265-284 |
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Authors: | van der Kraan Marieke I A van der Made Christel Nazmi Kamran van't Hof Wim Groenink Jasper Veerman Enno C I Bolscher Jan G M Nieuw Amerongen Arie V |
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Institution: | Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. |
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Abstract: | LFampin 265-284, derived from bovine lactoferrin, has broad-spectrum antimicrobial activity against the yeast Candida albicans and several Gram-positive and Gram-negative bacteria. A glycine substitution scan was used to identify residues that are important for its candidacidal activity. Each single substitution of a positively charged residue led to considerable reduction in candidacidal activity, for each residue to a different extent. Substitution within the helix-facilitating N-terminal sequence DLIW had less severe effect; substitution of Ile and Trp led to a somewhat reduced potency. No substantial effects were found on the propensity to adopt a helical structure or to bind to C. albicans cells. |
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