Chronic intrastriatal dopamine infusions in rats with unilateral lesions of the substantia nigra |
| |
| Authors: | R Hargraves W J Freed |
| |
| Institution: | 1. Fishery Engineering Department, Santa Catarina State University, Laguna 88790-000, Brazil;2. Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Federal University of Santa Catarina, Florianópolis 88034-257, Brazil;3. Aquaculture Pathology Research Center, Federal University of Santa Catarina, Florianópolis 88034-257, Brazil;4. Animal and Food Production Department, Santa Catarina State University, Lages 88520-000, Brazil;5. Department of Natural Sciences, Regional University of Blumenau, Blumenau 89012-170, Brazil;6. Oceanography and Limnology Department, Federal University of Maranhão, São Luís 65080-805, Brazil;7. Laboratory of Marine Mollusks, Federal University of Santa Catarina, Florianópolis 88034-257, Brazil;8. Department of Scientific and Technological Education, Santa Catarina State University, Florianópolis 88035-001, Brazil;1. Associate Professor, Department of Nursing, Inha University, Michuhol-gu, Incheon 22212, Republic of Korea;2. Professor, College of Nursing, Sahmyook University, Nowon-gu, Seoul, 01795, Republic of Korea |
| |
| Abstract: | This study examined the effects of continuously supplied dopamine delivered directly into the dopamine-deficient striatum. Rats received unilateral lesions of the substantia nigra by stereotaxic administration of 6-hydroxydopamine and were tested for apomorphine-induced rotational behavior and general activity. Osmotic mini-pumps were filled with dopamine in various concentrations, implanted subcutaneously and connected to a cannula implanted directly into the striatum. The system delivered solution at a rate of .5 microliter/hr for two weeks. Dopamine in a dosage of 0.5 microgram/per hour reduced apomorphine-induced rotational behavior by a mean of 52 +/- 5.8% (mean +/- SEM, n = 20) with a maximal individual decrease of 99%. There was no change in general activity or increase in stereotyped behavior. Infusions of vehicle solutions did not decrease rotational behavior. Spread of the infused dopamine and its metabolites was estimated by adding 3H-dopamine to the pumps in tracer quantities. Radioactivity was highly concentrated at the infusion site and decreased rapidly within a few mm from the infusion site. Continuous infusion methods may eventually prove to be effective in the treatment of nigro-striatal degenerative disease. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
