| 一种基于绿色荧光蛋白的蛋白酶体抑制剂细胞筛选模型 |
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| 引用本文： | 方海同,胡政,周光飚. 一种基于绿色荧光蛋白的蛋白酶体抑制剂细胞筛选模型[J]. 生物工程学报, 2009, 25(3): 452-456 |
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| 作者姓名： | 方海同 胡政 周光飚 |
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| 作者单位： | 1. 中国科学院广州生物医药与健康研究院,广州,510663;中国科学技术大学生命科学学院,合肥,230027
2. 中国科学院广州生物医药与健康研究院,广州,510663 |
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| 基金项目： | 国家自然科学基金项目(No.30871110);;中国科学院知识创新工程重要方向项目(No.KSCX1-YW-R-26);;广东省自然科学基金重点项目(No.06107503);;广东省科技计划项目(No.2007B030701005);;国家高技术研究发展计划(863计划)(No.2006AA02A301)资助~~ |
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| 摘 要： | 为建立基于绿色荧光蛋白(GFP)的药物筛选模型,并用此模型从包括中药提取物在内的化合物中筛选新型蛋白酶体抑制剂,本研究构建了pGC-E1-ZU1-GFP融合蛋白慢病毒表达载体并感染A549细胞,筛选稳定表达细胞株,用已知蛋白酶体抑制剂PS-341处理细胞,荧光显微镜检测处理前后细胞GFP水平变化。结果获得了稳定表达pGC-E1-ZU1-GFP的A549细胞,这些细胞用PS-341处理24h后用荧光显微镜检测,发现细胞绿色荧光强度相对于对照组明显增强。利用这一模型对一些化合物进行筛查,发现了一些新的蛋白酶体抑制剂。
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| 关 键 词： | 蛋白酶体抑制剂 筛选模型 绿色荧光蛋白 |
| 收稿时间： | 2008-09-04 |
Establishment of a GFP-based cellular model for screening novel proteasome inhibitors |
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| Haitong Fang,Zheng Hu and Guangbiao Zhou. Establishment of a GFP-based cellular model for screening novel proteasome inhibitors[J]. Chinese journal of biotechnology, 2009, 25(3): 452-456 |
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| Authors: | Haitong Fang Zheng Hu Guangbiao Zhou |
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| Affiliation: | Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China; School of Life Science, University of Science and Technology of China, Hefei 230027, China;Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China;Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China; School of Life Science, University of Science and Technology of China, Hefei 230027, China |
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| Abstract: | To establish a green fluorescent protein (GFP)-based cellular model for screening proteasome inhibitors from compounds including extracts from Traditional Chinese Medicinal herbs, we transfected A549 cells with lentivirus expression vector pGC-E1-ZU1-GFP, and selected clones stably expressing ZU1-GFP. The A549-ZU1-GFP cells were treated with PS-341 for 24 h, and the accumulation of GFP was analyzed by fluorescence microscope. We found that the fluorescence intensity of A549-ZU1-GFP cells treated with PS-341... |
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| Keywords: | proteasome inhibitor screening model green fluorescent protein |
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