Oxidative stress and inflammation in the normal airways and blood of patients with lung cancer and COPD |
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| Institution: | 1. Pulmonology Department, Muscle and Respiratory System Research Unit, IMIM–Institut Hospital del Mar, Parc de Salut Mar, and Health and Experimental Sciences Department, Universitat Pompeu Fabra, Universitat Autònoma de Barcelona, Parc de Recerca Biomèdica de Barcelona, E-08003 Barcelona, Spain;2. Centro de Investigación en Red de Enfermedades Respiratorias, Instituto de Salud Carlos III, Bunyola, Majorca, Balearic Islands, Spain;3. Pathology Department, IMIM–Institut Hospital del Mar, Parc de Salut Mar, Barcelona, Spain;3. From the Departments of Physiology and;4. Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140,;5. the Department of Biochemistry, University of Pavia, Pavia 27100, Italy, and;6. the Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan;1. Department of Pediatric Intensive Care Unit, Children''s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China;2. China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing, China;3. Chongqing Key Laboratory of Pediatrics, Chongqing, China;1. Anaesthesiological Investigations Unit, University Hospitals of Geneva, Geneva, Switzerland;2. Department of Medical Physics and Informatics, University of Szeged, Szeged, Hungary;3. Respiratory Medicine Department, Inselspital, University of Bern, Bern, Switzerland;4. Department of Paediatrics, University Hospitals of Geneva, Geneva, Switzerland;1. National and Kapodistrian University of Athens, Department of Physical Education and Sports Sciences, Athens, Greece;2. National and Kapodistrian University of Athens, 1st Department of Respiratory Medicine, Pulmonary Rehabilitation Unit, Sotiria Hospital, Athens, Greece;3. Technological Educational Institute of Central Greece, Department of Physiotherapy, Lamia, Greece;4. Cardiovascular Research Laboratory, Biomedical Research Foundation, Academy of Athens, Greece;5. National and Kapodistrian University of Athens, 1st Department of Critical Care Medicine, Evangelismos Hospital, Greece;1. Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition(PPABIONUT), Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, Abou-Bekr Belkaïd University, Tlemcen 13000, Algeria;2. Cardiology Department of Tlemcen University Hospital Center, Tlemcen 13000, Algeria;3. INSERM UMR 866, “Lipids Nutrition Cancer”, Faculty of Life, Earth, and Environment Sciences, University of Burgundy, Dijon 21000, France |
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| Abstract: | Respiratory conditions such as chronic obstructive pulmonary disease (COPD) are associated with a greater risk for lung cancer (LC). Oxidative stress and inflammation are involved in LC pathophysiology. Studies conducted so far have focused solely on lung tumor parenchyma and not the airways. We explored levels of local and systemic oxidative stress and inflammation within normal bronchial epithelium and blood of patients with lung cancer (n=52), with and without COPD, and in control subjects (COPD and non-COPD, n=21). In normal bronchial epithelium specimens (bronchoscopy) and blood from patients with similar smoking history (LC–COPD and LC) and control subjects (both COPD and non-COPD), redox balance and inflammatory markers were measured (ELISA and immunoblotting). All subjects were clinically evaluated. Absence of malignant cells within the bronchial specimens was always pathologically confirmed. Bronchial levels of protein carbonylation, MDA–protein adducts, antioxidants, TNF-α, interferon-γ, TGF-β, and VEGF and blood levels of superoxide anion, oxidatively damaged DNA and proteins, TNF-α, interferon-γ, TGF-β, VEGF, and neutrophils were significantly greater in all LC patients compared to control subjects. Systemic levels of oxidatively damaged DNA, superoxide anion, and TNF-α and bronchial levels of TGF-β and TNF-α showed high sensitivity and specificity for LC among patients. Regardless of the presence of an underlying respiratory condition (COPD), protein oxidation, oxidatively damaged DNA, and inflammation were remarkably increased in the normal airways and blood of patients with LC. Furthermore, the potential predictive value for LC development of these molecular events warrants attention and should be explored in future larger longitudinal studies. |
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| Keywords: | Oxidatively damaged DNA and proteins Normal bronchial epithelium Blood Lung cancer COPD Free radicals |
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