Evaluating biopulping as an alternative application on oil palm trunk using the white-rot fungus Trametes versicolor |
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| Affiliation: | 1. Division of Bio-resource, Paper and Coatings Technology, School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia;2. Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi 221005, India;1. School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM, 43600 Bangi, Selangor, Malaysia;2. Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, UKM, 43600 Bangi, Selangor, Malaysia;3. Department of Applied Microbiology, Faculty of Applied Sciences, Taiz University, 6803 Taiz, Yemen;4. Department of Chemical Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, UTM, Skudai, 81310 Johor, Malaysia;1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China;2. Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, PR China;1. Laboratory of Enzymology and Recombinant DNA Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak 124001, Haryana, India;2. Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, United States;3. School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh 201310, India;4. Academy of Microbiological Sciences, Green Woods City, Sector 46, Gurugram 122002, Haryana, India;1. Strategic Water Infrastructure Laboratory, School of Civil, Mining and Environmental Engineering, University of Wollongong (UOW), NSW 2522, Australia;2. Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan;3. Smart Water Research Centre, Australian Rivers Institute, School of Environment, Griffith University, QLD 4222, Australia;4. Strategic Water Infrastructure Laboratory, School of Chemistry, UOW, NSW 2522, Australia;1. College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, PR China;2. Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China |
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| Abstract: | The objective of the research was to investigate the suitability of the white-rot fungus Trametes versicolor for biopulping using oil palm biomass as the substrate. Fungi are grown on solid-state cultures Kirk's enrichment media to determine the lignocellulolytic activities. Samples subjected to fungal pretreatment for periods of 1, 2, 3, and 4 weeks were investigated and compared to the untreated control. The crude enzyme extracts were assayed using specific substrates and enzyme activities were calculated. The highest level of laccase activity was 218.66 U/L; the peak activity of manganese peroxidase was 162.10 U/L, and lignin peroxidase is 42.56 U/L. The activity levels of cellulase and hemicellulase were insignificant in all extracts (53.30 and 1.50 U/L, respectively). When the chips were pulped mechanically the Kappa number, pulp yield, and screened pulp yield decreased significantly and paper strength increased marginally with the exposure time. Hand sheet properties were also improved significantly by fungal treatment. Weight loss, lignin loss, cellulose, and holocellulose loss were 8.45%, 9.35%, 4.58%, and 7.2%, respectively. Images from SEM seem to indicate a simultaneous type of decay pattern involving cell wall breakdown combined with lignin modification. Considering all its pulping and papermaking properties, the performance of T. versicolor is good and has potential for use in large-scale biotechnological processes. |
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| Keywords: | Biopulping Lignocellulose Laccase Manganese peroxidase Lignin peroxidase |
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