Indigenous PAH-degrading bacteria from oil-polluted sediments in Caleta Cordova,Patagonia Argentina |
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| Institution: | 1. Department of Biology, College of Science, Sultan Qaboos University, Oman;2. Department of Marine Science and Fisheries, College of Agricultural and Marine Science, Sultan Qaboos University, Oman;1. Technical Unit for Environmental Characterization, Prevention, and Recovery ENEA, Via Anguillarese, 301, 00123 Rome, Italy;2. Department of Science and Chemical Technology, Tor Vergata University, Via della Ricerca Scientifica, 1 I-00133 Rome, Italy;3. Faculty of Mathematical, Physical and Natural Sciences, Sciences for the Conservation of Cultural Heritage, Firenze University, Viale Morgagni, 40/44, 50134 Firenze, Italy;4. Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Cagliari, Italy;1. Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada;2. School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK;1. School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, 710055 Shaanxi, Xi’an, China;2. Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, China;3. Key Laboratory of Environmental Engineering, Shaanxi Province, China;1. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic;2. Institute of Environmental Studies, Faculty of Science, Charles University, Benátská 2, CZ-128 01 Prague 2, Czech Republic |
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| Abstract: | Indigenous bacteria with the capability to degrade polycyclic aromatic hydrocarbons (PAH) were isolated from polluted sediment samples recovered from Caleta Cordova by using selective enrichment cultures supplemented with phenanthrene. Bacterial communities were evaluated by denaturing gradient gel electrophoresis (DGGE) in order to detect changes along enrichment culture and relationships with the representative strains subsequently isolated. Members of these communities included marine bacteria such as Lutibacter, Polaribacter, Arcobacter and Olleya, whose degradation pathway of PAH has not been studied yet. However, isolated bacteria obtained from this enrichment comprised the genus Pseudomonas, Marinobacter, Salinibacterium and Brevibacterium. The ability of isolates to grow and degrade naphthalene, phenanthrene and pyrene was demonstrated by detection of the residual substrate by HPLC. Archetypical naphthalene and catechol dioxygenase genes were found in two isolates belonging to genus Pseudomonas (Pseudomonas monteilii P26 and Pseudomonas xanthomarina N12), suggesting biodegradation potential in these sediments. The successful bacterial isolation with the ability to degrade PAH in pure culture suggest the possibility to study and further consider strategies like growth stimulation in situ, in order to increase the intrinsic bioremediation opportunities in the polluted Caleta Cordova harbor. |
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| Keywords: | Biodegradation PAH Caleta Cordova Polluted sediment C23O |
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