Biomarkers of oxidative stress study V: Ozone exposure of rats and its effect on lipids,proteins, and DNA in plasma and urine |
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| Affiliation: | 1. National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA;2. Faculty of Medicine, Uppsala University, Uppsala, Sweden;3. Biochemistry, Molecular Biology and Nutrition Department, University of Auvergne, Clermont-Ferrand, France;4. Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, USA;5. School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, UK;6. Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN, USA;7. The Heart Research Institute, University of Sydney, Sydney, Australia;8. Rush University Medical Center, Chicago, IL, USA;9. Northwest Life Science Specialties, LLC., Vancouver, WA, USA;10. Department of Pharmacology and Medicine, Vanderbilt University, Nashville, TN, USA;11. Children''s Hospital Oakland Research Institute, Oakland, CA, USA;12. University of Southern California, Los Angeles, CA, USA;13. Victor Chang Cardiac Research Institute, Darlinghurst, Australia;14. Otto-von-Guericke-University, Magdeburg, Germany;15. United States Environmental Protection Agency, Research Triangle Park, NC, USA;1. Hangzhou Zheda Dixun Biological Gene Engineering Co, Ltd, Hangzhou, China;2. Research Center of Allergy & Immunology of Shenzhen University School of Medicine, Shenzhen, China;3. Longgang ENT Hospital and the Shenzhen ENT Institute, Shenzhen, China;4. Department of Pediatric Otolaryngology, Shenzhen Hospital of Southern Medical University, Shenzhen, China, and Department of Otolaryngology, Guangzhou Women and Children''s Medical Center, Guangzhou Medical University, Guangzhou, China;5. Zhejiang Chinese Medical University, Hangzhou, China;1. Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, 4225 Roosevelt Way NE, Seattle, WA, 98105, USA;2. Department of Epidemiology, School of Public Health, University of Washington, 3980 15th Ave NE, Seattle, WA, 98195, USA;3. Department of Pathology and Laboratory Medicine, Department of Biochemistry, Larner College of Medicine, University of Vermont, 360 S. Park Drive, Colchester, VT, 05446, USA;4. Department of Medicine, School of Medicine, University of Washington, 4225 Roosevelt Way NE, Seattle, WA, 98105, USA;5. Department of Biostatistics, School of Public Health, University of Washington, 1705 NE Pacific St, Seattle, WA, 98195, USA;6. College of Pharmacy, University of New Mexico, MSC09 5360, 1 University of New Mexico, Albuquerque, NM, 87131, USA;1. Veterinary practice Đurđevac d.o.o., Croatia;2. Faculty of Veterinary Medicine University of Zagreb, Croatia |
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| Abstract: | Ozone exposure effect on free radical-catalyzed oxidation products of lipids, proteins, and DNA in the plasma and urine of rats was studied as a continuation of the international Biomarker of Oxidative Stress Study (BOSS) sponsored by NIEHS/NIH. The goal was to identify a biomarker for ozone-induced oxidative stress and to assess whether inconsistent results often reported in the literature might be due to the limitations of the available methods for measuring the various types of oxidative products. The time- and dose-dependent effects of ozone exposure on rat plasma lipid hydroperoxides, malondialdehyde, F2-isoprostanes, protein carbonyls, methionine oxidation, and tyrosine- and phenylalanine oxidation products, as well as urinary malondialdehyde and F2-isoprostanes were investigated with various techniques. The criterion used to recognize a marker in the model of ozone exposure was that a significant effect could be identified and measured in a biological fluid seen at both doses at more than one time point. No statistically significant differences between the experimental and the control groups at either ozone dose and time point studied could be identified in this study. Tissue samples were not included. Despite all the work accomplished in the BOSS study of ozone, no available product of oxidation in biological fluid has yet met the required criteria of being a biomarker. The current negative findings as a consequence of ozone exposure are of great importance, because they document that in complex systems, as the present in vivo experiment, the assays used may not provide meaningful data of ozone oxidation, especially in human studies. |
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| Keywords: | Biomarkers Oxidative stress Ozone exposure Rats |
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