Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments |
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| Institution: | 1. Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, Casilla de Correo 1245, 7600 Mar del Plata, Buenos Aires, Argentina;2. CNEA Comisión Nacional de Energía Atómica, Centro Atómico Constituyentes, Av. Gral Paz 1499, 1650 San Martín, Buenos Aires, Argentina;3. Instituto Nacional de Investigación y Desarrollo Pesquero, Casilla de Correo 175, 7600 Mar del Plata, Buenos Aires, Argentina;1. Laboratório Nacional Agropecuário (Lanagro-MG), Av. Rômulo Joviano, s/n, Pedro Leopoldo, MG CEP 33600-000, Brazil;2. Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, MG CEP 30123-970, Brazil;1. Department of Earth and Environmental Sciences, Korea University, Anam Dong 5-1, Seoul 136-701, Republic of Korea;2. Department of Mathematics, Korea University, Anam Dong 5-1, Seoul 136-701, Republic of Korea;3. Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea;1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. Department of Chemistry, Nanchang University, Nanchang 330031, China;3. School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane 4001, Australia;1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin 300072, PR China;2. Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, PR China;3. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, PR China |
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| Abstract: | Samborombón Bay, Argentina, is a highly productive area exposed to chronic contamination, including polycyclic aromatic hydrocarbons. Four phenanthrene-degrading strains were isolated from sediments collected in this area. Analysis of partial 16S rRNA sequencing and a BLAST search indicated that three of the strains belong to genus Pseudomonas and one to Sphingomonas. All the strains were able to grow in 150 mg l−1 phenanthrene as the sole carbon and energy source, with high degradation efficiency (75–100% in 72–168 h). Growth in sodium salicylate indicated that the Pseudomonas strains used this pathway to degrade phenanthrene.Strategies that may enhance substrate bioavailability, such as surfactant production and chemotactic responses, were tested. Two Pseudomonas strains showed significant production of surface-active compounds, and a strong chemotactic response toward phenanthrene. Together with the ability to consume the supplied phenanthrene to completion, these characteristics make the mentioned strains good candidates for bioremediation strategies intended to clean up polluted areas. |
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