Change in cytosolic calmodulin activity of density (age) separated human erythrocytes towards membrane Ca2+/Mg2+ ATPase |
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Authors: | J E Ekholm S D Shukla D J Hanahan |
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Institution: | Department of Biochemistry The University of Texas Health Science Center San Antonio, Texas 78284 USA |
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Abstract: | The membrane ATPase of density (age) separated human erythrocytes was examined for its stimulation by the cytosols of these cell groups. On the assumption that the stimulatory activity in the cytosol is only calmodulin, it was consistently observed that the young cytosol had a significantly higher activity towards the membrane ATPase activity (from any age group) than did the old cell cytosol. The data clearly demonstrates decided differences in the expression of calmodulin activity in cytosols from young and old erythrocytes and would support the conclusion that calmodulin activity is altered during aging of these cells. Possible mechanisms for these alterations are discussed. |
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Keywords: | NaCl-His buffer 0 155 M NaCl 5 mM histidine pH 7 6 |
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