Identification and Analysis of Proteins Sharing Dehydroascorbate Reductase Activity |
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Authors: | MC De Tullio C Paciolla O Arrigoni |
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Institution: | (1) Dipartimento di Biologia e Patologia Vegetale, Università di Bari, Via E. Orabona 4, I-70125 Bari, Italia |
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Abstract: | Dehydroascorbate (DHA)-reducing proteins were observed using a native-PAGE/activity staining method, and identified in soybean and rice by Western analysis using antibodies against homologous protein known to catalyse DHA reduction. Administration of 1 mM DHA apparently did not trigger DHA reductase activity in lupin and onion, whereas activity was increased in rice and barley. |
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Keywords: | Allium cepa ascorbate regeneration Glycine max Lupinus albus Oryza sativa |
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